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Glycitin

Synonyms: Glycitein-7-β-O-glucoside

Glycitin (Glycitein-7-β-O-glucoside), a natural isoflavone isolated from legumes, has antibacterial, antiviral and estrogenic activities and may exerts preventative effects on alcoholism, cardiovascular and cerebrovascular diseases and some types of cancer.

Glycitin Chemical Structure

Glycitin Chemical Structure

CAS: 40246-10-4

Purity & Quality Control

Batch: S382501 DMSO] 89 mg/mL] false] ] ] false] ] ] false Purity: 99.99%
99.99

Glycitin Related Products

Biological Activity

Description Glycitin (Glycitein-7-β-O-glucoside), a natural isoflavone isolated from legumes, has antibacterial, antiviral and estrogenic activities and may exerts preventative effects on alcoholism, cardiovascular and cerebrovascular diseases and some types of cancer.
In vitro
In vitro Glycitin promotes cell proliferation, osteoblast induction, and activates Col I mRNA expression and ALP activity of BMSCs. Glycitin promotes the proliferation and migration of human dermal fibroblast cells through TGF-β and p-AKT signaling[1].
Cell Research Cell lines Bone marrow stem cells (BMSCs)
Concentrations 0.01, 0.5, 1, 5 and 10 µM
Incubation Time 7 days
Method BMSCs (1-2×106 cells or 1-2×104 per well) are cultured in 6- or 96-well culture plates overnight at 37°C in an atmosphere containing 5% CO2. Glycitin is added to the wells at final concentrations of 0.01, 0.5, 1, 5 and 10 µM and cultured for 7 days. In cells cultured in 6-well culture plates, BMSCs are determined using Oil Red O staining and observed via light microscopy at 510 nm. BMSCs are fixed using 5% precooled paraformaldehyde for 30 min at 4°C and stained with 0.6% (w/v) Oil Red O solution for 15 min at room temperature. Cells stained with Oil Red O are washed with water (3×5 min) to remove unbound dye, and culture dishes are stained with 1 ml isopropyl alcohol for 10 min. In cells cultured in 96-well culture plates, BMSCs are determined via MTT assay. A total of 20 µl MTT (5 g/l) are added to each well and cultured for 4 h. The supernatant is removed and 200 µl dimethylsulfoxide are added to each well for 15 min. Optical density (OD) is measured using a microplate spectrophotometer at 570 nm. Proliferation rate is calculated using: OD treated / OD control × 100%.
In Vivo
In vivo Glycitin significantly prevents bone loss in variectomized (ovx) rats at a dose of 50 mg/kg/d. At this dose glycitin also prevents ovx-induced uterine atrophy and increases in body weight gain, abdominal fat, serum total cholesterol and triglyceride, and urinary excretion of pyridinoline and deoxypyridinoline with statistical significance[2].
Animal Research Animal Models Female Sprague-Dawley rats
Dosages 25, 50 or 100 mg/kg/d
Administration oral

Chemical Information & Solubility

Molecular Weight 446.40 Formula

C22H22O10
CAS No. 40246-10-4 SDF Download Glycitin SDF
Smiles COC1=C(C=C2C(=C1)C(=O)C(=CO2)C3=CC=C(C=C3)O)OC4C(C(C(C(O4)CO)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 89 mg/mL ( (199.37 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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