Home Immunology & Inflammation Immunology & Inflammation related inhibitor Ganoderic acid A For research use only.

Ganoderic acid A

Ganoderic acid A (GAA), a representative active triterpenoid from Ganoderma lucidum, has been reported to exhibit antinociceptive, antioxidative, cytotoxic, hepatoprotective and anticancer activities.

Ganoderic acid A Chemical Structure

Ganoderic acid A Chemical Structure

CAS: 81907-62-2

Purity & Quality Control

Batch: S475301 DMSO] 100 mg/mL] false] Ethanol] 100 mg/mL] false] Water] Insoluble] false Purity: 99.78%
99.78

Ganoderic acid A Related Products

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Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Bel7402 cells Cytotoxicity assay Cytotoxicity against human Bel7402 cells, IC50=7.25 μM 23092389
P388 cells Cytotoxicity assay Cytotoxicity against mouse P388 cells, IC50=7.25 μM 23092389
SGC7901 cells Cytotoxicity assay Cytotoxicity against human SGC7901 cells, IC50=7.25 μM 23092389
HepG2 cells Function assay 48 h Anticancer activity against human HepG2 cells assessed as cell viability after 48 hrs by MTT assay, IC50=38.7 μM 24974349
Click to View More Cell Line Experimental Data

Biological Activity

Description Ganoderic acid A (GAA), a representative active triterpenoid from Ganoderma lucidum, has been reported to exhibit antinociceptive, antioxidative, cytotoxic, hepatoprotective and anticancer activities.
In vitro
In vitro GAA exhibits antitumor activity on human osteosarcoma, lymphoma, meningioma and breast cancer cells through suppressing growth and invasive behavior and/or inducing apoptosis of cancer cells. GAA could also enhance chemosensitivity of HepG2 cells to Cisplatin[1]. GA-A treatment induces caspase-dependent apoptotic cell death characterized by a dose-dependent increase in active caspases 9 and 3, up-regulation of pro-apoptotic BIM and BAX proteins, and a subsequent loss of mitochondrial membrane potential with release of cytochrome c. Lower doses of GA-A enhance HLA class II-mediated antigen presentation and CD4+ T cell recognition of lymphoma in vitro[2].
Cell Research Cell lines Human pre-B acute lymphocytic leukemia (NALM-6), Burkitt lymphoma (Ramos, GA-10, CA-46 and Daudi) and non-Hodgkin
Concentrations 5, 10, 20, and 40μM
Incubation Time 24 h
Method

Cells are seeded at 1×104 cells/well in 100μl of appropriate culture medium in a flat-bottom 96-well plate. GA-A is added to appropriate wells for final concentrations of 5, 10, 20, and 40μM. Following 24h of GA-A treatment, cell viability is measured using the CellTiter 96 AQueous One Solution Cell Proliferation Assay (MTS).
In Vivo
In vivo GAA can significantly prolong the survival of EL4 syngeneic mice and decrease tumor metastasis to the liver, and enhance cell-mediated immune responses by attenuating myeloid-derived suppressor cells. GAA could undergo extensive metabolism, including reduction, oxidation, and hydroxylation phase I metabolism, and glucuronidation and sulfation phase II metabolism[1].
Animal Research Animal Models Male Sprague-Dawley rats
Dosages 20 mg/kg
Administration i.v.

Chemical Information & Solubility

Molecular Weight 516.67 Formula

C30H44O7
CAS No. 81907-62-2 SDF Download Ganoderic acid A SDF
Smiles CC(CC(=O)CC(C)C(=O)O)C1CC(C2(C1(CC(=O)C3=C2C(CC4C3(CCC(=O)C4(C)C)C)O)C)C)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (193.54 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 100 mg/mL

Water : Insoluble


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In vivo
Batch:

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In vivo Formulation Calculator

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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