Escin

Synonyms: Aescin

Escin (Aescin) is a mixture of saponins with anti-inflammatory, vasoconstrictor and vasoprotective effects found in Aesculus hippocastanum(the horse chestnut).

Escin Chemical Structure

Escin Chemical Structure

CAS: 6805-41-0

Purity & Quality Control

Batch: Purity: 100.00%
100.00

Escin Related Products

Choose Selective Immunology & Inflammation related Inhibitors

Biological Activity

Description Escin (Aescin) is a mixture of saponins with anti-inflammatory, vasoconstrictor and vasoprotective effects found in Aesculus hippocastanum(the horse chestnut).
In vitro
In vitro Escin is effective to inhibit pancreatic cancer cell proliferation as a single agent. However, escin has minimal effect on normal rat skeletal muscle cells. Escin potentiates the proliferation-inhibiting and apoptosis-inducing effect of gemcitabine in both BxPC-3 and PANC-1 cell lines in vitro. It also down-regulates constitutive as well as gemcitabine-induced activation of NF-kB and its downstream gene products in pancreatic cancer cells in vitro[1].
Cell Research Cell lines The human pancreatic cancer cell lines BxPC-3, PANC-1
Concentrations 25 μM (BxPC-3), 20 μM (PANC-1)
Incubation Time 72 h
Method Cell cycle analysis: After treatment with escin (BxPC-3 (25 μM),PANC-1 (20 μM),respectively), gemcitabine (BxPC-3(100 nM), PANC-1(1 μM), respectively) or their combinations for 72 h, BxPC-3 and PANC-1 cells are collected by trypsinization, washed with ice-cold PBS and fixed overnight by the addition of 2 ml of ice-cold 70% ethanol/30% PBS at 4°C. The ethanol is subsequently removed after centrifugation, and about 1×106 cells are resuspended in 800 μl of PBS, 100 μl of ribonuclease A (500 g/ml PBS) and 100 μl of PI (500 g/ml PBS) in the dark at room temperature for 30 min. Flow cytometric analysis is performed using FACScan for the detection of the percentage of cells in the diVerent phases of the cell cycle. Cells treated with DMSO alone are used as controls.
In Vivo
In vivo Escin augments therapeutic effect of gemcitabine in BxPC-3 xenografts in nude mice. They have significant synergistic effects on suppressing the growth of pancreatic tumors, inhibiting the proliferation and inducing apoptosis of tumors. Escin potentiates the inhibiting effect of gemcitabine on NF-kB and NF-kB-regulated gene products in BxPC-3 xenografts in nude mice[1].
Animal Research Animal Models BALB/c nude mice
Dosages 2 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 1131.26 Formula

C55H86O24

CAS No. 6805-41-0 SDF Download Escin SDF
Smiles CC=C(C)C(=O)OC1C(C2(C(CC1(C)C)C3=CCC4C5(CCC(C(C5CCC4(C3(CC2O)C)C)(C)CO)OC6C(C(C(C(O6)C(=O)O)OC7C(C(C(C(O7)CO)O)O)O)O)OC8C(C(C(C(O8)CO)O)O)O)C)CO)OC(=O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 100 mg/mL ( (88.39 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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