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Eflornithine (DFMO) hydrochloride hydrate

Name: Eflornithine (DFMO) hydrochloride hydrate
Brand: Selleck Chemicals
SKU: S4582
Rating: 5 (8 reviews)

Synonyms: Difluoromethylornithine hydrochloride hydrate, MDL-71782 hydrochloride hydrate, RMI-71782 hydrochloride hydrate, α-difluoromethylornithine hydrochloride hydrate

Catalog No.S4582 Purity: 97%

Eflornithine (DFMO) hydrochloride hydrate inhibits polyamine biosynthesis by the selective, irreversible inhibition of ornithine decarboxylase (ODC). A chemoprotective agent that blocks angiogenesis. Its biological half-life is 8 hours.

Eflornithine (DFMO) hydrochloride hydrate Chemical Structure

CAS: 96020-91-6

Selleck's Eflornithine (DFMO) hydrochloride hydrate has been cited by 8 publications

Purity & Quality Control

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Choose Selective Decarboxylase Inhibitors

Cell Data

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
J774.1	Antitrypanosomal assay	48 hrs	Antitrypanosomal activity against Trypanosoma brucei brucei TC221 infected in mouse J774.1 cells after 48 hrs by alamar blue assay, IC50 = 22.9 μM.	22376072
A673	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
NB1643	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
SK-N-MC	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
Click to View More Cell Line Experimental Data

Biological Activity

Description

Targets

Orn decarboxylase ^[2]

In vitro
In vitro	When cultured cells are treated with α-difluoromethyl-Orn, an inhibitor of polyamine biosynthesis, production of hydrogen peroxide is suppressed and programmed cell death did not occur^[1].
Cell Research	Cell lines	BY2 cell
	Concentrations	2 mM
	Incubation Time	48 h
	Method	BY2 cells are treated with or without cryptogein in the presence or absence of DFMO(Difluoromethylornithine) and monitered the effect of chemicals on cell growth.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	c-Myc / α-tubulin		25248858
	Growth inhibition assay	2T1 cells		21093499
	IHC	H&E staining of pancreas p21 / PCNA		25248858
	IHC/IF	ODC expression in pancreatic tumors Cav-1 / β-catenin		25248858

In Vivo
In vivo	Eflornithine alters changes in vascular responsiveness associated with coarctation hypertension
Animal Research	Animal Models	Rats
	Dosages	400 mg/kg
	Administration	s.c.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05594563	Recruiting	Type 1 Diabetes	Emily K. Sims\|Juvenile Diabetes Research Foundation\|Cancer Prevention Pharmaceuticals Inc.\|Indiana University	March 14 2023	Phase 2
NCT05500508	Recruiting	Cancer\|Solid Tumor\|Solid Carcinoma\|Advanced Cancer\|DIPG Brain Tumor\|Ovary Cancer\|Breast Cancer\|Papillary Thyroid Cancer\|Head and Neck Cancer\|Gastric Cancer\|Nsclc\|Mesotheliomas Pleural\|Mesothelioma Peritoneum\|Esophageal Cancer\|Diffuse Midline Glioma H3 K27M-Mutant\|Endometrial Cancer\|Cervical Cancer\|Melanoma\|Colorectal Cancer\|Glioma Malignant	Aminex Therapeutics Inc.	November 29 2022	Phase 1\|Phase 2
NCT04696029	Recruiting	Medulloblastoma	Giselle Sholler\|Milton S. Hershey Medical Center	March 29 2021	Phase 2
NCT03536728	Completed	Cancer\|Solid Tumor\|Solid Carcinoma\|Advanced Cancer	Aminex Therapeutics Inc.	June 12 2018	Phase 1
NCT02636569	Active not recruiting	Non-melanoma Skin Cancer	University of Alabama at Birmingham	October 25 2017	Phase 2

References

Chemical Information & Solubility

Molecular Weight	236.64	Formula	C₆H₁₂F₂N₂O₂.HCl.H₂O
CAS No.	96020-91-6	SDF	Download Eflornithine (DFMO) hydrochloride hydrate SDF
Smiles	C(CC(C(F)F)(C(=O)O)N)CN.O.Cl
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

Water : 47 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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