Home GPCR & G Protein GPCR19 activator Deoxycholic acid For research use only.

Deoxycholic acid

Synonyms: Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic

Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity. Deoxycholic acid is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.

Deoxycholic acid Chemical Structure

Deoxycholic acid Chemical Structure

CAS: 83-44-3

Selleck's Deoxycholic acid has been cited by 1 publication

Purity & Quality Control

Batch: S468901 DMSO] 78 mg/mL] false] Ethanol] 60 mg/mL] false] Water] Insoluble] false Purity: 99.94%
99.94

Deoxycholic acid Related Products

Choose Selective GPCR19 Inhibitors

Biological Activity

Description Deoxycholic acid (Deoxycholate, Desoxycholic acid, Cholerebic, Cholorebic) is a cytolytic agent. The physiologic effect of deoxycholic acid is by means of decreased cell membrane integrity. Deoxycholic acid is specifically responsible for activating the G protein-coupled bile acid receptor TGR5 that stimulates brown adipose tissue (BAT) thermogenic activity.
In vitro
In vitro Deoxycholic acid inhibits miR-21 expression in primary rat hepatocytes in a dose-dependent manner, and increases miR-21 pro-apoptotic target programmed cell death 4 (PDCD4) and apoptosis. Deoxycholic acid decreases NF-κB activity, shown to represent an upstream mechanism leading to modulation of the miR-21/PDCD4 pathway[1].
Cell Research Cell lines Primary rat hepatocytes
Concentrations 25 to 200 μM
Incubation Time 24 h
Method

Primary rat hepatocytes were isolated from male rats (100 to 150 g) by collagenase perfusion. After isolation, hepatocytes were resuspended in complete Williams E medium and plated on BD Primaria™ culture dishes at 5 × 104 cells/cm2. Cells are kept at 37 °C in a humidified atmosphere of 5% CO2 for 4-6 h to allow attachment. Plates are then washed with phosphate buffered saline (PBS) 1× in order to remove dead cells and incubated in Williams E medium supplemented with 25 to 200 μM DCA or no addition (control) for 24 h. Primary rat hepatocytes are processed for total RNA and protein isolation, cell viability, cytotoxicity and caspase activity assays and Hoechst staining.
In Vivo
In vivo miR-21 expression is down-regulated upon short exposures to Deoxycholic acid, as is the downstream pathway, with concomitant PIDD processing and activation of caspase-2, which contributes to DCA-induced liver damage[1].
Animal Research Animal Models Male Wistar rats
Dosages 125 mg/kg
Administration p.o.
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02654496 Completed
Obesity
North Dakota State University
 January 2016 --
NCT01865812 Completed
Primary Biliary Cirrhosis
Intercept Pharmaceuticals
 December 3 2013 Phase 2
NCT01319916 Completed
Healthy
Kythera Biopharmaceuticals
 December 2010 Phase 1

Chemical Information & Solubility

Molecular Weight 392.57 Formula

C24H40O4
CAS No. 83-44-3 SDF Download Deoxycholic acid SDF
Smiles CC(CCC(=O)O)C1CCC2C1(C(CC3C2CCC4C3(CCC(C4)O)C)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 78 mg/mL ( (198.69 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 60 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Deoxycholic acid | Deoxycholic acid ic50 | Deoxycholic acid price | Deoxycholic acid cost | Deoxycholic acid solubility dmso | Deoxycholic acid purchase | Deoxycholic acid manufacturer | Deoxycholic acid research buy | Deoxycholic acid order | Deoxycholic acid mouse | Deoxycholic acid chemical structure | Deoxycholic acid mw | Deoxycholic acid molecular weight | Deoxycholic acid datasheet | Deoxycholic acid supplier | Deoxycholic acid in vitro | Deoxycholic acid cell line | Deoxycholic acid concentration | Deoxycholic acid nmr