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Colchicine

Name: Colchicine
Brand: Selleck Chemicals
SKU: S2284
Rating: 5 (19 reviews)

Synonyms: Colchineos, Colchisol, Colcin,NSC 757

Catalog No.S2284 Purity: 99.99%

Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM.

Colchicine Chemical Structure

CAS: 64-86-8

Selleck's Colchicine has been cited by 19 publications

Purity & Quality Control

Batch: Purity: 99.99%

99.99

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Signaling Pathway

Microtubule Associated Signaling Pathway

Choose Selective Microtubule Associated Inhibitors

Biological Activity

Description	Colchicine is a microtubule polymerization inhibitor with an IC50 of 3 nM.

In vitro
In vitro	Colchicine is a tubulin inhibitor and a microtubule disrupting agent, which inhibits the tubulin polymerization on the site of β-tubulin.^[1]
Cell Research	Cell lines	HeLa cells
	Concentrations	5 μM
	Incubation Time	2 h
	Method	HeLa cells are cultured in 6-well plates and then treated with or without different concentrations of KXO1, vinblastine, or colchicine for 2 h before being treated with 100 μM EBI for another 2 h. Total protein is extracted with radioimmuno_x0002_precipitation assay buffer and subjected to Western blot analy_x0002_sis of β-tubulin. GAPDH is used as a loading control. The Western blotting procedure is performed.

In Vivo
In vivo	Colchicine prevents NSAID-induced small intestinal injury by inhibiting activation of the NLR family pyrin domain-containing 3 (NLRP3) inflammasome.^[2]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05855746	Not yet recruiting	Acute Myocarditis	Assistance Publique - Hôpitaux de Paris\|Hospices Civils de Lyon\|Fonds de Dotation ACTION	January 22 2024	Phase 3
NCT06020300	Recruiting	STEMI	National University of Malaysia	July 28 2023	Phase 4
NCT05472337	Recruiting	Acute Coronary Syndrome	Pontificia Universidad Catolica de Chile\|Fondo Nacional de Desarrollo Científico y Tecnológico Chile	August 1 2022	Phase 2

References

[1] Niu L, et al. J Biol Chem. 2019 Nov 29;294(48):18099-18108.

Chemical Information & Solubility

Molecular Weight	399.44	Formula	C₂₂H₂₅NO₆
CAS No.	64-86-8	SDF	Download Colchicine SDF
Smiles	CC(=O)NC1CCC2=CC(=C(C(=C2C3=CC=C(C(=O)C=C13)OC)OC)OC)OC
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 80 mg/mL ( (200.28 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 80 mg/mL

Water : 79 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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