Chloroquine Phosphate

Catalog No.S4157

Chloroquine Phosphate Chemical Structure

Molecular Weight(MW): 515.86

Chloroquine phosphate is a 4-aminoquinoline anti-malarial and anti-rheumatoid agent, also acting as an ATM activator.

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3 Customer Reviews

  • NCI-H929 EV and miR-137 OE cells were treated with ATM activator Chloroquine Phosphate (CQ), specific ATM inhibitor KU-55933, and ATR inhibitor AZ20 for 12 hr. Immunoblotting showed the expression of p-ATM, p-Chk2, p-BRCA1, p-ATR and p-Chk1.

    Leukemia, 2017. Chloroquine Phosphate purchased from Selleck.

    Cells were pre-treated with rapamycin (Rapa, 1 μM) for 12 h, or KRIBB3 (KR, 0.5 μM) or chloroquine (CQ, 10 μM) for 2 h, followed by treatment with of palmitate (PA, 150 μM) for 24 h. Then the lipid in L02 cells was observed with Oil Red O (ORO) staining and ORO absorbance detection. Values are means ± SD (n = 3), *p b 0.05, versus matched controls.

    Cell Signal, 2016, 28(8):1086-98. . Chloroquine Phosphate purchased from Selleck.

  • Rapamycin aggravates the autophagic reaction induced by CVB3 infection. HeLa cells were treated with 10 nM rapamycin and chloroquine phosphate (CQ group) for 2 h. The cells were washed thrice and infected with CVB3. In the CVB3 group, HeLa cells were incubated with DMEM containing 10% FBS for 2 h and infected with CVB3. The sham group was incubated with DMEM containing 10% FBS. Two hours later, the medium was refreshed with DMEM containing 2% FBS. At 24 h.p.i., cells were collected for western blot analysis. The LC3-II/LC3-I ratio was increased in the CVB3 and rapamycin group (p<0.01), and p-mTOR was decreased in the CVB3 group (p<0.05) and rapamycin group (p<0.01) compared with the sham group. The changes in the LC3-II/LC3-I ratio and p-mTOR were more obvious in the Rap group compared with the CVB3 group (p<0.05). The LC3-II/LC3-I ratio and p62 was increased in the CQ group compared with the CVB3 group (p<0.05). *p<0.05, compared with the sham group; **p<0.01, compared with the sham group; #p<0.05, compared with the CVB3 group. CVB3, coxsackievirus B3; DMEM, Dulbecco's modified Eagle's medium; FBS, fetal bovine serum; h.p.i., hours post-inoculation; LC3, light chain 3; mTOR, mammalian target of rapamycin.

    Int J Mol Med, 2017. Chloroquine Phosphate purchased from Selleck.

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Biological Activity

Description Chloroquine phosphate is a 4-aminoquinoline anti-malarial and anti-rheumatoid agent, also acting as an ATM activator.
Targets
ATM [1]
(Cell-free assay)
In vitro

Chloroquine is a chemotherapeutic agent for the clinical treatment of malaria. Chloroquine is able to bind to DNA, and inhibit DNA replication and RNA synthesis which in turn results in cell death. The effect of Chloroquine may also be related to the formation of a toxic heme-Chloroquine complex. Chloroquine inhibits trophozoite hemoglobin degradation through increasing vacuolar pH and inhibiting the activity of vacuolar phospholipase, vacuolar proteases, and heme polymerase[1]. Chloroquine possesses definite antirheumatic properties. Chloroquine has immuno-modulatory effects, suppressing the production/release of tumour necrosis factor and interleukin 6. Moreover, Chloroquine exerts direct antiviral effects, inhibiting pH-dependent steps of the replication of several viruses including members of the flaviviruses, retroviruses, and coronaviruses. Its best-studied effects are those against HIV replication[2]. Chloroquine can accumulate inside the macrophage phagolysosome by ion trapping where it exerts potent antifungal activity against Histoplasma capsulatum and Cryptococcus neoformans by distinct mechanisms. Chloroquine inhibits growth of H. capsulatum by pH-dependent iron deprivation, whereas it is directly toxic to C. neoformans[3].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human K562 cells MkjkR5l1d3SxeHnjxsBie3OjeR?= Ml\SR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT|U3OiClZXzsd{BjgSCPVGSgZZN{[XluIFnDOVA:OzFwOEOgcm0> NVexUo92OTh3M{i1Olc>
human KB cells NWnEWGJlS3m2b4TvfIlkyqCjc4PhfS=> NHrKR2pEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBMSiClZXzsd{BjgSCvaXPyc5Bt[XSnIH3leIhw\CxiSVO1NF0xNjZizszN MmjFNVQ3PDB3MkS=
human MDA-MB-468 cells M4jaUmN6fG:2b4jpZ:Kh[XO|YYm= MlnaOFghcA>? MUPDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNE[4JINmdGy|IHHmeIVzKDR6IHjyd{BjgSCVUlKgZZN{[XliaX6gdJJme2WwY3Wgc4YhOy53IIXNJFQuMDJvY3jsc5JwNTFySD3wbIVvd3ijennuMVExNXmuKT3OMG4u\GmndHj5cIJ2fGGwLUGtZY1qdmViaInkdo9kcGyxcnnk[UwhT0l3ME2yMlgyKM7:TR?= MY[xPVk1PTF7Nx?=
human MCF7 cells MXHDfZRwfG:6aXRCpIF{e2G7 MVW0PEBp MknRR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUWNHPyClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG|c3H5JIlvKHC{ZYPlcoNmKG:oIECuOEB2VSBzLTixMUg1NSh5LYDo[Y56dC1zSD3pcYll[XqxW{SsOU1oZXG3aX7vfIFtcW5vNj35cElj\W68eXypdIlx\XKrZHnuMVQugWxrLUHIMYJmdnqxW3TdbY1q\GG8b3ytNkg{UClvb37lMEBIUTVyPUWuOVEh|ryP Mon5NVk6PDVzOUe=
human MDA-MB-231 cells Ml;lR5l1d3SxeHnjxsBie3OjeR?= NELmcWY1QCCq NF:3T3NEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IES4JIhzeyCkeTDTVmIh[XO|YYmgbY4heHKnc3XuZ4Uhd2ZiMz61JJVOKDRvKEKtZ4htd3KxLUGwTE1xcGWwb4jhfolvNTFyLYnsLU1PNE5vZHnleIh6dGK3dHHuMVEu[W2rbnWgbJllem:laHzvdoll\SxiR1m1NF03NjB6IN88US=> NEn3ZpcyQTl2NUG5Oy=>
human HeLa cells Ml:zR5l1d3SxeHnjxsBie3OjeR?= M3fNWWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMh[XO|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25iYomgUXRVKGG|c3H5MEBKSzVyPUOwJO69VQ>? MU[yOFM2PDN{Mh?=
human HepG2 cells NFv5WYxEgXSxdH;4bYPDqGG|c3H5 NVTUdmZ2PDhiaB?= MUHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[ZBIOiClZXzsd{Bi\nSncjC0PEBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUO3MlY5KM7:TR?= NUfXN|lROjN6MUWxPFY>

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Protocol

Solubility (25°C)

In vitro Water 100 mg/mL (193.85 mM)
DMSO Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 515.86
Formula

C18H26ClN3.2H3O4P

CAS No. 50-63-5
Storage powder
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02511054 Completed Malaria National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) July 21, 2015 Phase 1
NCT02432417 Not yet recruiting Glioblastoma|Astrocytoma, Grade IV Maastricht Radiation Oncology January 2018 Phase 2
NCT02932007 Recruiting Atrial Fibrillation University of South Florida January 2017 Phase 2
NCT02773979 Recruiting Plasmodium Falciparum Infection National Institute of Allergy and Infectious Diseases (NIAID) September 2016 Phase 1
NCT02563496 Not yet recruiting Malaria, Vivax GlaxoSmithKline|Medicines for Malaria Venture September 2016 Phase 2
NCT02930343 Recruiting Rheumatoid Arthritis Jawaharlal Institute of Postgraduate Medical Education & Research September 2016 Phase 4

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID