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BrdU (Bromodeoxyuridine)

Name: BrdU (Bromodeoxyuridine)
Brand: Selleck Chemicals
SKU: S7918
Rating: 5 (11 reviews)

Synonyms: 5-Bromo-2'-deoxyuridine, BUdR

Catalog No.S7918 Purity: 99.99%

BrdU (Bromodeoxyuridine) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.

BrdU (Bromodeoxyuridine) Chemical Structure

CAS: 59-14-3

Selleck's BrdU (Bromodeoxyuridine) has been cited by 11 Publications

3 Customer Reviews

Purity & Quality Control

Batch: Purity: 99.99%

99.99

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Signaling Pathway

DNA/RNA Synthesis Signaling Pathway

Choose Selective DNA/RNA Synthesis Inhibitors

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
mouse neural precursor cells	Antiproliferative assay	Antiproliferative activity against mouse neural precursor cells by MTT assay, EC50=2.045μM	17417631
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of FdUrd resistant L5178Y-Resistant murine leukemia cells, IC50=12μM	11101356
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]Leu., IC50=20μM	11101356
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of L5178Y-Parental murine leukemia cells, IC50=26μM	11101356
murine leukemia cells	Growth inhibition assay	In vitro growth inhibition of L5178Y-Parental murine leukemia cells by incorporation of [14C]-Thd., IC50=26μM	11101356
Click to View More Cell Line Experimental Data

Biological Activity

Description	BrdU (Bromodeoxyuridine) is a nucleoside analog that competes with thymidine for incorporation into DNA, and used in the detection of proliferating cells.

In vitro
In vitro	In RG2 rat glioma cells, Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion. In H9 cells and BJ fibroblasts, Bromodeoxyuridine alters the cell cycle profile. ^[1] BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession. ^[2]
Cell Research	Cell lines	RG2 rat glioma cells
	Concentrations	~ 50 µM
	Incubation Time	24 hours
	Method	Cultures are initially plated at 2000 cells/cm² and are quantified with a Z2 Coulter Counter. RG2 rat glioma cells are treated once with 0, 1, 10, or 50 µM BrdU for 24 hours, and cumulative growth curves were obtained over 18 days. Control and treated cells are quantified and replated at equal densities on days 5, 12, and 18 after treatment.

In Vivo
In vivo	In rat glioma RG2 tumor model, Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/ml, p.o.) significantly slows tumor progression. ^[1]

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT04365205	Recruiting	Asthma	University Hospital Bordeaux	September 23 2020	--
NCT02654925	Completed	Obesity	University of Colorado Denver	March 2016	--
NCT01407211	Unknown status	Relapsing Remitting Multiple Sclerosis	Tehran University of Medical Sciences	April 2011	Phase 4
NCT01414972	Unknown status	Atherosclerosis	Tehran University of Medical Sciences	May 2010	Phase 4
NCT01225289	Completed	Relapsing Remitting Multiple Sclerosis	Tehran University of Medical Sciences	October 2009	Phase 4
NCT00032344	Completed	Colorectal Cancer	US Department of Veterans Affairs\|VA Office of Research and Development	October 1993	Phase 3

References

Chemical Information & Solubility

Molecular Weight	307.1	Formula	C₉H₁₁BrN₂O₅
CAS No.	59-14-3	SDF	Download BrdU (Bromodeoxyuridine) SDF
Smiles	C1C(C(OC1N2C=C(C(=O)NC2=O)Br)CO)O
Storage (From the date of receipt)	3 years -20°C(in the dark) powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C(in the dark) powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 61 mg/mL ( (198.63 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.					In vivo Formulation Calculator
	Clear solution	4%DMSO 1 Corn oil	7.5mg/ml (24.42mM)	Taking the 1 mL working solution as an example, add 40 μL of 187.5 mg/ml clear DMSO stock solution to 960 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.	In vivo Formulation Calculator
Clear solution	5% DMSO 1 95% corn oil	0.5mg/ml (1.63mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O	10.0mg/ml (32.56mM)	Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clarified DMSO stock solution to 400 μL PEG300, mix evenly to clarify it; add 50 μL Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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