Berbamine (dihydrochloride)

Catalog No.S3609

Berbamine (dihydrochloride) Chemical Structure

Molecular Weight(MW): 681.65

Berbamine (BBM) is a natural bisbenzylisoquinoline product isolated from traditional Chinese herbal medicine Berberis amurensis. It is a novel inhibitor of bcr/abl fusion gene with potent anti-leukemia activity and also an inhibitor of NF-κB.

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Biological Activity

Description Berbamine (BBM) is a natural bisbenzylisoquinoline product isolated from traditional Chinese herbal medicine Berberis amurensis. It is a novel inhibitor of bcr/abl fusion gene with potent anti-leukemia activity and also an inhibitor of NF-κB.
In vitro

Berbamine (BBM) potently suppresses liver cancer cell proliferation and induces cancer cell death by targeting Ca2+/calmodulin-dependent protein kinase II (CAMKII). Through targeting CAMKII, BBM's inhibition on cancer cells might have broader effects beyond affecting JAK/STAT3 and p210bcr-abl, because CAMKIIγ directly impacts many other signal pathways including STAT1, NF-κB, JNK, ERK1/2, FOXO1, and Wnt/β-catenin[1]. BBM can effectively inhibit tumor metastasis by suppressing cell proliferation, migration and invasion in highly metastatic breast cancer cells under in vitro condition[2]. Berbamine inhibits proliferation of K562-r cells both in vitro and in vivo. Berbamine-induced apoptosis in K562-r cells appear to occur through a mechanism involving Bcl-2 family proteins, as well as mdr-1 mRNA and P-gp protein. Berbamine in combination with imatinib restore the chemo-sensitivity of K562-r cells to imatinib[3].

In vivo BBM inhibits the in vivo tumorigenicity of liver cancer cells in NOD/SCID mice, and down-regulated the self-renewal abilities of liver cancer initiating cells[1]. BBM shows its anticancer activity by induction of apoptosis, cell cycle arrest and reversing multidrug resistance. Though BBM is a potent drug but its half-life in blood plasma is very short, owing to its quick renal clearance[2].

Protocol

Cell Research:

[1]

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  • Cell lines: epithelial liver cancer cell lines, including Huh7, HepG2, MHCC97H, and PLC/PRF/5
  • Concentrations: 0-80 μg/ml
  • Incubation Time: 72 h
  • Method:

    Cell proliferation is assayed 72 hours after BBM treatment for liver cancer cells with epithelial morphology.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: NOD/SCID mouse
  • Formulation: pure sterile water
  • Dosages: 100 mg/kg
  • Administration: oral
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (146.7 mM)
Water 100 mg/mL (146.7 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 681.65
Formula

C37H40N2O6.2ClH

CAS No. 6078-17-7
Storage powder
in solvent
Synonyms N/A

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID