Anacardic Acid

Catalog No.S7582 Synonyms: 6-pentadecylsalicylic Acid

Anacardic Acid Chemical Structure

Molecular Weight(MW): 348.52

Anacardic Acid is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition.

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  • Western blot analysis of α-SMA expression in whole-lung lysates. Data are expressed as means ± SEM, *P < 0.05 versus vehicle and #P < 0.05 versus PQ (n = 4-6).

    Front Immunol, 2017, 7:696. Anacardic Acid purchased from Selleck.

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Biological Activity

Description Anacardic Acid is a potent inhibitor of p300 and p300/CBP-associated factor histone acetyltranferases, which also has antibacterial activity, antimicrobial activity, prostaglandin synthase inhibition, and tyrosinase and lipoxygenase inhibition.
Targets
Tip60 [2]
(Cell-free assay)
PCAF(p300/CREB-binding protein-associated factor) [1]
(Cell-free assay)
p300/CBP [1]
(Cell-free assay)
5 μM 8.5 μM
In vitro

Anacardic acid is found to be a common inhibitor of several clinically targeted enzymes such as NFkB kinase, histone acetyltransferase (HATs), lipoxygenase (LOX-1), xanthine oxidase, tyrosinase and ureases[4]. Anacardic acid is also an efficient inhibitor of Tip60 in vitro.It inhibits the Tip60-dependent acetylation and activation of the ATM protein kinase in HeLa cells, and sensitizes the cells to the cytotoxic effects of radiation[2]. Anacardic acid can effectively prevent cell damage induced by H2O2 because this can be converted to ROS, hydroxyl radicals, in the presence of metal ions[4].

In vivo Anacardic acid attenuates over-expression of Gata4, α-MHC and cTnT in fetal mouse hearts exposed to ethanol. Anacardic acid inhibits binding of P300, PCAF to the promoter of Gata4[3].

Protocol

Kinase Assay:[3]
+ Expand

HAT and HDAC Activity Assays :

After homogenization of cardiac tissues, nucleoproteins are extracted using a Nuclear Extract Kit according to the manufacturer's instructions. HAT and HDAC activities of the nuclear protein extracts are determined using a colorimetric assay included in the HAT and HDAC assay kits.
Cell Research:[2]
+ Expand
  • Cell lines: HeLa and 293T cells, SQ20B and SCC35 squamous cell carcinoma cell lines
  • Concentrations: 0-100 μM
  • Incubation Time: 40 min
  • Method: HeLa and 293T cells are grown in Dulbecco’s modified Eagle’s medium/10% fetal calf serum. SQ20B and SCC35 squamous cell carcinoma cell lines are cultured in modified eagles medium/10% fetal calf serum.For anacardic acid (EMD Biosciences, CA) exposure, cells are preincubated with anacardic acid for 40 min, irradiated, then allowed to recover for 4 h. Cells are then switched to fresh media and allowed to grow for 10 days.
    (Only for Reference)
Animal Research:[3]
+ Expand
  • Animal Models: 9 to 11 week old Kunming mice
  • Formulation: Dissolved in DMSO, co-injected with ethanol.
  • Dosages: 0, 1.25, 2.5, 5, 10 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 69 mg/mL (197.98 mM)
Ethanol 69 mg/mL warmed (197.98 mM)
Water Insoluble
In vivo Add solvents individually and in order:
5% DMSO+45% PEG 300+H2O
8mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 348.52
Formula

C22H36O3

CAS No. 16611-84-0
Storage powder
Synonyms 6-pentadecylsalicylic Acid

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Related Antibodies

Histone Acetyltransferase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID