A-769662

Catalog No.S2697

A-769662 Chemical Structure

Molecular Weight(MW): 360.39

A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.

Size Price Stock Quantity  
In DMSO USD 210 In stock
USD 147 In stock
USD 270 In stock
USD 470 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

4 Customer Reviews

  • Cells were treated with CP, DOXO, SRT1720 (100 nM), EX527 (100 nM), A769662 (10 μM) and Compound C (1 μM) under normoxic or hypoxic conditions for 48 hours, and then their viabilities were measured by MTT.

    Cancer Res 2014 74(1), 298-308. A-769662 purchased from Selleck.

    Eight- to 10-week-old Ldlr-/- mice fed a standard laboratory chow were fasted overnight, fed at 07:00 h for 2 h, and refasted at 09:00 h. Intraperitoneal injection of vehicle, GW1516 (3 mg/kg), or A-769662 (30 mg/kg) (n = 6/group) occurred at the beginning of the refasting period at 09:00 h. Immunoblots of AMPK and ACC in freeze-clamped liver lysates 90 min postinjection. Representative immunoblots with quantitations are shown. Asterisk (*) indicates significant difference between vehicle and treatment; Student's paired t-test (P < 0.05).

    J Lipid Res 2014 55(7), 1254-1266. A-769662 purchased from Selleck.

  • Concentration-dependent effect of synthetic AMPK stimulator, A-769662, applied for 10 min on phosphorylated α subunit of AMPK. AMPK inhibitor (compound C, CC) was added to some samples at 10 uM. **p < 0.01, ***p < 0.001 vs. sample without A-769662 by ANOVA and Tukey post hoc test.

    Pharmacol Res 2014 81, 34-43. A-769662 purchased from Selleck.

    Cultured PANC-1 pancreatic cancer cells were treated with vehicle (DMSO, 1%), 10 uM of belinostat (BS, for 2 h, 4 h and 6 h) or A-769662 (10 uM, 2 h), phospho- and total AMPK and ACC were detected by western blot, tubulin (loading control) was also tested. AMPK and ACC phosphorylation was quantified as described.

    Biochem Biophys Res Commun 2013 437(1), 1-6. A-769662 purchased from Selleck.

Purity & Quality Control

Choose Selective AMPK Inhibitors

Biological Activity

Description A-769662 is a potent, reversible AMPK activator with EC50 of 0.8 μM in cell-free assays, little effect on GPPase/FBPase activity.
Targets
AMPK [1]
(Cell-free assay)
Fatty acid synthesis [1]
(in primary rat hepatocytes)
0.8 μM(EC50) 3.2 μM
In vitro

A-769662 stimulates partially purified rat liver AMPK with EC50 with 0.8 μM. A-769662 activates AMPK purified from multiple tissues and species in a dose-responsive manner with modest variations in observed EC50s. EC50s determined for A-769662 using partially purified AMPK extracts from rat heart, rat muscle, or human embryonic kidney cells (HEKs) are 2.2 mM, 1.9 mM, or 1.1 mM, respectively. A 4 hours treatment of primary rat hepatocytes with A-769662 dose-dependently increases ACC phosphorylation, which correlated inhibition of fatty acid synthesis with IC50 of 3.2 μM. A-769662 also inhibits fatty acid sythesis in mouse hepatocytes with IC50 with 3.6 μM [1] A-769662 activates AMPK both allosterically and by inhibiting dephosphorylation of AMPK on Thr-172, similar to the effects of AMP. [2] A-769662 inhibits proteasomal function by an AMPK-independent mechanism. A-769662 affects the in vitro activity of purified 26S proteasomes but not the in vitro activity of purified 20S proteasomes. A-769662 has toxic effects on MEF cells. [3] A recent research shows A-769662 inhibited cell proliferation and DNA synthesis. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
mouse hepatocytes NVjSbHdSTnWwY4Tpc44h[XO|YYm= MVqxJI1O NVrBZ5c{TE2VTx?= NFjvbI5qdmirYnn0d{Bn[XS2eTDhZ4llKHO7boTo[ZNqeyC5aYToJGlEPTBib3[gN{43KM7:TR?= MnjYNVY4PTN3N{[=
rat hepatocytes NULK[nV6TnWwY4Tpc44h[XO|YYm= NHm1eXMyKG2P NHfnXJZFVVOR MnnVbY5pcWKrdIOg[oF1fHliYXPp[EB{gW62aHXzbZMhf2m2aDDJR|UxKG:oIEOuOkDPxE1? NEDRZ5EyPjd3M{W3Oi=>
HEK293 NGTlbJZMcW6jc3WgZZN{[Xl? NXywRZFEOjByIN88US=> M37mNGROW09? MnPxZYN1cX[jdHXzJIVv\G:pZX7veZMhSU2SSx?= NWfyeYVDOTd5MkiyOFE>
CCL13 M{\jc2tqdmG|ZTDhd5NigQ>? MYWyNFAh|ryP MXfEUXNQ NUj0cGJL[WO2aY\heIV{KGWwZH;n[Y5wfXNiQV3QTy=> NUntbVVrOTd5MkiyOFE>
MEFs NWfsVnNMTnWwY4Tpc44h[XO|YYm= MmruN|AxKM7:TR?= M3rCWWROW09? MUHpcohq[mm2czDwdo91\WG|b33hcEBnfW6ldHnvckBjgSCjbjDBUXBMNWmwZHXw[Y5l\W62IH3lZ4hidmm|bR?= MkXONVg2QTN3OES=
epididymal clear cells M4j6VWZ2dmO2aX;uJIF{e2G7 NIrQNHkzODBizszN M{WxcmROW09? NVfOd3pKcW6qaXLpeJMhfGinIIDIMY1m\GmjdHXkJHYuSVSSYYPlJIFk[3WvdXzheIlwdiCjdDD0bIUh[XCrY3HsJI1mdWK{YX7l NUDDTXBxOTl{MUG5NVg>
3T3-L1 NH\wd41HfW6ldHnvckBie3OjeR?= MV2xMlIhdU1? Mn\nSG1UVw>? NWq1fI5OcW6qaXLpeJMhO1R|LVyxJGFlcXCxZ3Xu[ZNqew>? M4TkelE6PDh|M{C0
3T3-L1 NXHZU2I5TnWwY4Tpc44h[XO|YYm= NGT0e3kyNjJibV2= MWnEUXNQ MUHpcohq[mm2czD0bIUhTXiycnXzd4lwdiCxZjDB[Ilxd2enbnXzbZNT\WyjdHXkJHRz[W6|Y4LpdJRqd25iRnHjeI9zeyCjbnSgUYFzc2W{cx?= MX[xPVQ5OzNyNB?=
3T3-L1 MkTiSpVv[3Srb36gZZN{[Xl? MYSxMlIhdU1? MXXEUXNQ NGj6Z4RqdmirYnn0d{BOcXSxdHnjJGNtd26jbDDFfJBidnOrb36= MoP3NVk1QDN|MES=
3T3-L1 NEfsbHZkgXSxdH;4bYNqfHliYYPzZZk> NYfHfVF{OS5{IH3N NHTFc5RFVVOR MX\k[YNz\WG|ZYOgR4VtdCCYaXHibYxqfHl? M13i[FE6PDh|M{C0
3T3-L1 Ml21T4lv[XOnIHHzd4F6 Mke5NU4zKG2P MWnEUXNQ NGfGOlVi[3SrdnH0[ZMhSU2SSx?= M3:ybVE6PDh|M{C0
L6 skeletal muscle cells NWnYW2JYTnWwY4Tpc44h[XO|YYm= M2fTR|I2OCEQvF2= MlTTSG1UVw>? M4DZUIFkfGm4YYTld{BCVVCNIIPp[45idGmwZzDwZZRpf2G7cx?= NEC0VpoyQTh{OEizOi=>
L6 skeletal muscle cells MoTzSpVv[3Srb36gZZN{[Xl? MkD3NlUxKM7:TR?= Moe4SG1UVw>? M{PKVIlvcGmkaYTzJJRp\SCQYTutT{suSVSSYYPlJJRz[W6|cH;yeEBi[3Srdnn0fUBidmRiY3XscEB{fXKoYXPlJIFjfW6mYX7j[S=> M2jQblE6QDJ6OEO2
MDA-MB231 M1vv[mFxd3C2b4Ppd{Bie3OjeR?= NWqxSHg5PDByIN88US=> M1nsR2ROW09? Mk[5d4Vve2m2aYrld{BpfW2jbjDidoVie3RiY3HuZ4VzKGOnbHygcIlv\XNidH:gWHJCUUxvaX7keYNm\CCjcH;weI9{cXN? MoTXNVk5QTZ2Nkm=
BT474 M1H5[GFxd3C2b4Ppd{Bie3OjeR?= MVG0NFAh|ryP MWTEUXNQ NEjzOWp{\W6|aYTpfoV{KGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscEBtcW6nczD0c{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orcx?= MWmxPVg6PjR4OR?=
MCF7 MmjJRZBweHSxc3nzJIF{e2G7 M{HNb|QxOCEQvF2= MWTEUXNQ NEL2Wmx{\W6|aYTpfoV{KGi3bXHuJIJz\WG|dDDjZY5k\XJiY3XscEBtcW6nczD0c{BVWkGLTD3pcoR2[2WmIHHwc5B1d3Orcx?= NFTOVGsyQTh7NkS2PS=>
Mesenchymal stem cells MnjYT4lv[XOnIHHzd4F6 MnzuNVAhyrWP MlfnSG1UVw>? NVjE[pdCcW6mdXPld{BiKHKxYoXzeEBidmRic4XzeIFqdmWmIFHNVGsh[WO2aY\heIlwdg>? M1TpV|I1OTB2OEe5
Mesenchymal stem cells NVfteY1X[3m2b4TvfIlkcXS7IHHzd4F6 MoDaNVAxKML3TR?= M2PPe2ROW09? Ml\J[IVkemWjc3XzJJRp\SCPU1OgdJJwdGmoZYLheIlwdg>? MnLoNlQyODR6N{m=
MG-63 Ml3lZ5l1d3SxeHnjbZR6KGG|c3H5 M3rRRVExKML3TR?= MVTEUXNQ MnvxbY5pcWKrdIOgTFJQOi2LbnT1Z4VlKE:|dHXvZoxie3RiQ3XscEBF\WG2aB?= NYnKU45VOjR7NkCzOlI>
MC3T3-E1 MYnjfZRwfG:6aXPpeJkh[XO|YYm= M4jxe|ExKML3TR?= MX7EUXNQ NHy5[WRqdmirYnn0d{BJOk9{LVnu[JVk\WRiT4P0[Y9jdGG|dDDD[YxtKESnYYTo MnP6NlQ6PjB|NkK=
MG-63 M3XlVmFxd3C2b4Ppd{Bie3OjeR?= NFP1b4IyOCEEtV2= MnvhSG1UVw>? M1X6bZN2eHC{ZYPz[ZMhUDKRMj3JcoR2[2WmIF;zeIVw[myjc4SgR4VtdCCDcH;weI9{cXN? MmTNNlQ6PjB|NkK=
MC3T3-E1 MnjnRZBweHSxc3nzJIF{e2G7 MkP1NVAhyrWP M1HiRmROW09? NUn6bVhoe3WycILld5NmeyCKMl:yMWlv\HWlZXSgU5N1\W:kbHHzeEBE\WyuIFHwc5B1d3Orcx?= M1nDdlI1QTZyM{[y
MG-63 MVfGeY5kfGmxbjDhd5NigQ>? NHLsUGUyOCEEtV2= M1ywVmROW09? NIjX[WlidGyndnnheIV{KFKRUzDhZ4N2dXWuYYTpc44h[W6mIFHUVEBl\XCuZYTpc44h[2G3c3XkJIJ6KEh{T{K= MYOyOFk3ODN4Mh?=
MC3T3-E1 M13OeGZ2dmO2aX;uJIF{e2G7 NXvjSmFNOTBiwsXN M1LyTWROW09? Mm\mZYxt\X[rYYTld{BTV1NiYXPjeY12dGG2aX;uJIFv\CCDVGCg[IVxdGW2aX;uJINifXOnZDDifUBJOk9{ MYqyOFk3ODN4Mh?=
MG-63 MnvvSpVv[3Srb36gZZN{[Xl? NEDZOYkyOCEEtV2= MkjpSG1UVw>? Mo\i[oFkcWyrdHH0[ZMhUDKRMj3pcoR2[2WmIHH1eI9xcGGpeTDhZ5RqfmG2aX;u MUeyOFk3ODN4Mh?=
MC3T3-E1 NHrKVWJHfW6ldHnvckBie3OjeR?= M2O5V|ExKML3TR?= NFjqUIVFVVOR NEDCRldn[WOrbHn0ZZRmeyCKMl:yMYlv\HWlZXSgZZV1d3CqYXf5JIFkfGm4YYTpc44> M3nNT|I1QTZyM{[y
PC3 NWnNOlFMU2mwYYPlJIF{e2G7 MnLiNVAxKML3TR?= MXjEUXNQ NF7POHF2eHKnZ4XsZZRmeyC2aHWgcIV3\Wy|IH;mJGFOWEtiYX7kJGFESyCyaH;zdIhwenmuYYTpc44> M3P4WlI2PTl2MESz
PC3M MVXLbY5ie2ViYYPzZZk> Mn7GNVAxKML3TR?= NWrLWWszTE2VTx?= NV3pbHJbfXC{ZXf1cIF1\XNidHjlJIxmfmWuczDv[kBCVVCNIHHu[EBCS0NicHjvd5Bpd3K7bHH0bY9v MXyyOVU6PDB2Mx?=
PC3 M2XIWmZ2dmO2aX;uJIF{e2G7 M{KzdlExOCEEtV2= M2fr[GROW09? M1\SSIlv\HWlZYOgVGk{Uy:vVF;SJJBifGi5YYnz NX;PR5NzOjV3OUSwOFM>
PC3M Mlf5SpVv[3Srb36gZZN{[Xl? M{TEb|ExOCEEtV2= NXTmWJZQTE2VTx?= MoLMbY5lfWOnczDQTVNMN22WT2KgdIF1cHejeYO= MVeyOVU6PDB2Mx?=
PC3 MYHHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? M1LQd|ExOCEEtV2= NIjzVJRFVVOR NWTWNJVpe3WycILld5NmeyCycn;sbYZmemG2aX;u M3vjdVI2PTl2MESz
PC3M M4TIUWdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NFvoXIQyODBiwsXN NWezXmc2TE2VTx?= Mk\Md5VxeHKnc4Pld{Bxem:uaX\ldoF1cW:w NUPnN2FqOjV3OUSwOFM>
MC3T3-E1 MlnsT4lv[XOnIHHzd4F6 Mlv0NVAh|ryP NVGwdJRuTE2VTx?= Mo\nbY5lfWOnczDzbYdvcW[rY3HueEBCVVCNIHHjeIl3[XSrb36= Moq2NlY5QTF6Nk[=
MC3T3-E1 NUPneldsT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NE\CRlcyOCEQvF2= NV7OUVNiTE2VTx?= MV\pcohq[mm2czDE[ZgucW6mdXPl[EBwe3Snb3LsZZN1KGOnbHyg[IVifGh? NYTMNnRHOjZ6OUG4OlY>
MC3T3-E1 NVrRelFMTnWwY4Tpc44h[XO|YYm= NWHrWXk5OTBizszN NHewdVRFVVOR MoHIbY5pcWKrdIOgSIV5NWmwZIXj[YQhd3irZHH0bZZmKHO2cnXzdy=> M1PidFI3QDlzOE[2

... Click to View More Cell Line Experimental Data

In vivo Short-term treatment of normal Sprague Dawley rats with A-769662 decreases liver malonyl CoA levels and the respiratory exchange ratio, VCO2/VO2, indicating an increased rate of whole-body fatty acid oxidation. Treatment of ob/ob mice with 30 mg/kg b.i.d. A-769662 decreases hepatic expression of PEPCK, G6Pase, and FAS, lowers plasma glucose by 40%, reduced body weight gain and significantly decreases both plasma and liver triglyceride levels. [1]

Protocol

Kinase Assay:

[1]

+ Expand

96-well AMPK assay:

AMPK activity is measured by monitoring phosphorylation of the SAMS peptide substrate (20 mM in standard assays and 100 mM in additivity assays) following a previously described protocol (Anderson et al., 2004). To determine whether A-769662-induced AMPK activation occurs in a reversible manner, AMP or A-769662 are preincubated with rat liver AMPK for 10 minutes at 20 times standard assay concentrations prior to dilution and measurement of AMPK activity.
Cell Research:

[3]

+ Expand
  • Cell lines: MEF cells
  • Concentrations: 300 μM
  • Incubation Time: 24 hours
  • Method:

    Cell viability of MEF cells treated or not with A-769662 is performed as follows: cells are harvested by trypsinization and incubated with 0.5 mg/mL RNase and 50 μg/mL propidium iodine at room temperature in the dark; cell viability is analyzed by flow cytometry using a FACScanto flow cytometer, using an excitation laser at 488 nm and a propidium iodine fluorescence detection at 600 nm. To determine the proportion of cells in each phase of the cell cycle, cells are harvested by trypsinization, collected by centrifugation, washed in PBS and fixed overnight in 80% ethanol at -20 °C. Subsequently, these fixed cells are centrifuged to remove the fixative and incubated for 20 minutes in the dark at room temperature in PBS containing 0.5 mg/mL RNase and 50 μg/mL propidium iodine. Flow cytometry analysis is performed as above. The proportion of cells in G1, S, and G2 is determined using the MODFIT program. Cell culture pictures are taken at the indicated times using a camera coupled to an inverted microscope with a 20 × objective.


    (Only for Reference)
Animal Research:

[1]

+ Expand
  • Animal Models: Sprague Dawley rats
  • Formulation: A-769662 is dissolved in DMSO.
  • Dosages: 30 mg/kg
  • Administration: Administered via i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL (199.78 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents individually and in order:
1% DMSO+30% polyethylene glycol+1% Tween 80
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 360.39
Formula

C20H12N2O3S

CAS No. 844499-71-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

AMPK Signaling Pathway Map

Related AMPK Products

Tags: buy A-769662 | A-769662 supplier | purchase A-769662 | A-769662 cost | A-769662 manufacturer | order A-769662 | A-769662 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID