Name: Sulfanilamide
Brand: Selleck Chemicals
SKU: S1685
Rating: 5 (1 reviews)

Sulfanilamide (Sulphanilamide) is a competitive inhibitor for bacterial enzyme dihydropteroate synthetase with IC50 of 320 μM.

Sulfanilamide Chemical Structure

CAS: 63-74-1

Purity & Quality Control

Batch: S168501 DMSO] 34 mg/mL] false] Ethanol] 17 mg/mL] false] Water] Insoluble] false Purity: 99.95%

99.95

Sulfanilamide Related Products

Related Targets	tuberculosis	Click to Expand
Related Products	Berberine BTZ043 Racemate Moxifloxacin Amoxicillin Vancomycin HCl Econazole Teicoplanin Chloroxine Amoxicillin Sodium Dalbavancin Auranofin Ornidazole Proanthocyanidins Ethambutol 2HCl Colistin Sulfate Skatole Amoxicillin trihydrate Berberine Sulfate Bismuth Subcitrate Potassium Demeclocycline HCl Delamanid Bedaquiline fumarate Clindamycin HCl Pipemidic acid Ceftiofur Oxytetracycline hydrochloride Tetracycline Tobramycin sulfate Oxytetracycline Dihydrate Lefamulin acetate (Xenleta) PBTZ169 Cefepime Dihydrochloride Monohydrate Amikacin Sulfate Salt Aminothiazole Furagin Furaltadone HCl Nimorazole Trigonelline Hydrochloride Methyl α-D-mannopyranoside Chloramphenicol sodium succinate Ciprofloxacin hydrochloride hydrate Amikacin disulfate Furazolidone Nitrofurantoin Artemisic acid Pefloxacin Mesylate Protionamide 6-Methoxydihydrosanguinarine	Click to Expand
Related Compound Libraries	Anti-infection Compound Library Antibiotics compound Library Antiviral Compound Library Anti-parasitic Compound Library Gut Microbial Metabolite Library	Click to Expand

Choose Selective Bacterial Inhibitors

Biological Activity

Description

Sulfanilamide (Sulphanilamide) is a competitive inhibitor for bacterial enzyme dihydropteroate synthetase with IC50 of 320 μM.

Targets

dihydropteroate synthetase ^[1]

320 μM

In vitro
In vitro	Sulfanilamide containing the sulfonamide functional group displays inhibitory activity for dihydropteroate synthetase partially purified from Escherichia coli which normally uses para-aminobenzoic acid (PABA) for synthesizing the necessary folic acid acting as a coenzyme in the synthesis of purine, pyrimidine and other amino acids, exhibiting an IC 50 of 320 μM for dihydropteroate synthetasea and Km of 2.5 uM for PABA. ^[1] Sulfanilamide shows IC50 of 286.8 μg/mL for recombinant S. cerevisiae strains with wild-type FOL1 genes, but the single mutation ⁵⁵Trp to ⁵⁵Ala or ⁵⁷Pro to ⁵⁷Ser within the putative active site of the fungal DHPS confers resistance to Sulfanilamide with IC50 of >800 μg/mL. ^[2] Sulfanilamide moderately inhibits the growth of bacterial cells harboring plasmodium falciparum pKOS-pfPPPK-DHPS (His) with IC50 of 380 uM. ^[3]

In Vivo
In vivo	Administration of Sulfanilamide with the dosage of 100 mg/kg/day is effective in the prevention of P. carinii infection in the immunosuppressed rat model. When the dosage of sulfaguanidine and Sulfanilamide reduced to 10 mg/kg/day, breakthrough P. carinii infection occurs in the rats. ^[4]
Animal Research	Animal Models	Male Sprague-Dawley rats
	Dosages	100 mg/kg
	Administration	Orally taken every day

References

[4] Hughes WT, et al. Antimicrob Agents Chemother, 1996, 40(4), 962-965.

+ Expand

Chemical Information & Solubility

Molecular Weight	172.2	Formula	C₆H₈N₂O₂S
CAS No.	63-74-1	SDF	Download Sulfanilamide SDF
Smiles	C1=CC(=CC=C1N)S(=O)(=O)N
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 34 mg/mL ( (197.44 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 17 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):