Home Transmembrane Transporters NKCC inhibitor Furosemide For research use only.

Furosemide

Synonyms: NSC 269420

Furosemide is a potent NKCC2 (Na-K-2Cl symporter) inhibitor, used in the treatment of congestive heart failure and edema.

Furosemide Chemical Structure

Furosemide Chemical Structure

CAS: 54-31-9

Selleck's Furosemide has been cited by 5 publications

Purity & Quality Control

Batch: Purity: 99.96%
99.96

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Biological Activity

Description Furosemide is a potent NKCC2 (Na-K-2Cl symporter) inhibitor, used in the treatment of congestive heart failure and edema.
Targets
NKCC2 [4]
In vitro
In vitro

Furosemide reversibly alters the responses to tones and clicks of the chinchilla basilar membrane in hair cells, causing response-magnitude reductions that are largest (up to 61 dB, averaging 25-30 dB) at low stimulus intensities at the characteristic frequency (CF) and small or nonexistent at high intensities and at frequencies far removed from CF. Furosemide also induces response-phase lags that are largest at low stimulus intensities (averaging 77 degrees) and are confined to frequencies close to CF. [1]

Furosemide concentration- and time-dependently increases the formation of nitric oxide andprostacyclin. Furosemide leads to an enhanced release of kinins into the supernatant of the cells. [2]

Furosemide reversibly suppresses low Ca2+-induced epileptiform activity in hippocampus proper and blocks or significantly reduces different types of epileptiform discharges in the low Mg2+ model and the 4-aminopyridine model. [3]

Furosemide significantly inhibits cell growth in MKN45 cells, but not in MKN28 cells. Furosemide diminishes cell growth by delaying the G(1)-S phase progression in poorly differentiated gastric adenocarcinoma cells, which show high expression and activity of NKCC, but not in moderately differentiated gastric adenocarcinoma cells with low expression and NKCC activity. [4]
Cell Research Cell lines Erythrocytes
Concentrations 10, 30 and 100 µM
Incubation Time 45 minutes
Method

Cells were treated with indicated concentrations of drug for 45 minutes.
In Vivo
In vivo

Furosemide (0.25 to 2 mg/kg), administered 4 hours before exercise, reduces right atrial pressure (RAP) and pulmonary arterial pressure (PAP) during exercise in dose-dependent manner in horse. [5]
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06105255 Active not recruiting
Healthy Male
InventisBio Co. Ltd
 May 24 2023 Phase 1
NCT05915286 Recruiting
End Stage Renal Disease on Dialysis
University of Alberta
 May 29 2023 Phase 4
NCT05881603 Recruiting
Cardiogenic Acute Pulmonary Edema
Centre Hospitalier Universitaire de la Réunion
 May 20 2023 --
NCT05809011 Recruiting
Acute Heart Failure
Azienda Socio Sanitaria Territoriale degli Spedali Civili di Brescia
 April 24 2023 Phase 2|Phase 3

Chemical Information & Solubility

Molecular Weight 330.74 Formula

C12H11ClN2O5S
CAS No. 54-31-9 SDF Download Furosemide SDF
Smiles C1=COC(=C1)CNC2=CC(=C(C=C2C(=O)O)S(=O)(=O)N)Cl
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 66 mg/mL ( (199.55 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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