Home Metabolism Dehydrogenase inhibitor 3-Nitropropionic acid (3-NP) For research use only.

3-Nitropropionic acid (3-NP)

Synonyms: β-Nitropropionic Acid

3-Nitropropionic acid (3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.

3-Nitropropionic acid (3-NP) Chemical Structure

3-Nitropropionic acid (3-NP) Chemical Structure

CAS: 504-88-1

Selleck's 3-Nitropropionic acid (3-NP) has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.99%
99.99

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Biological Activity

Description 3-Nitropropionic acid (3-NP) is an irreversible inhibitor of mitochondrial respiratory Complex II succinate dehydrogenase, resulting in energy depletion through disruption of the electron transport chain.
Targets
Succinate dehydrogenase [1]
In vitro
In vitro 3-nitropropionic acid (3-NPA) is an irreversible inhibitor of complex II in the mitochondria. It impairs cellular energy metabolism via inhibition of succinate dehydrogenase, which induces a reduction in ATP production and leads to oxidative stress. 3-NPA can cause the generation and release of ROS from mitochondria, mitochondrial DNA damage, and thus loss of mitochondrial function. 3-NPA reduces the number of large follicles, impairs oocyte development, and increases the percentage of atretic large follicles and ROS levels in oocytes and granulosa cells[2]. ROS production followed by mitochondrial DNA damage is the primary event in 3-NPA toxicity, and Bcl-2 protects PC12 cells from 3-NPA toxicity by preventing mitochondrial DNA damage[3].
Cell Research Cell lines PC12 cells
Concentrations 4-8 mM
Incubation Time 48 h
Method Viability of the PC12 cells with or without treatment of 3-NPA is estimated using trypan blue exclusion test. Cells are grown in Petri dishes to a density of 1 × 106 cells/dish. After treatment with 4 or 8 mM 3-NPA for 48 h, cells are washed with Hank's balanced salt solution. Trypan blue (0.4 %) is added to the cells and incubated for 5 min. Both viable and nonviable cells are scored on a hemocytometer using a Nikon microscope. The nonviable cells are expressed as a percentage of total cell population
In Vivo
In vivo 3-NPA induces granulosa cell apoptosis, large follicle atresia, and an increase of ROS levels in the ovary[2].
Animal Research Animal Models ICR mice
Dosages 6.25, 12.5 and 25 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 119.08 Formula

C3H5NO4
CAS No. 504-88-1 SDF Download 3-Nitropropionic acid (3-NP) SDF
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 23 mg/mL ( (193.14 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 23 mg/mL

Ethanol : 23 mg/mL


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In vivo
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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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