Home Cytoskeletal Signaling PKC inhibitor 2-Methoxy-1,4-naphthoquinone For research use only.

2-Methoxy-1,4-naphthoquinone

2-Methoxy-1,4-naphthoquinone, isolated from the leaves of Impatiens glandulifera, specifically suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells.

2-Methoxy-1,4-naphthoquinone Chemical Structure

2-Methoxy-1,4-naphthoquinone Chemical Structure

CAS: 2348-82-5

Purity & Quality Control

Batch: S366101 DMSO] 37 mg/mL] false] Ethanol] 3 mg/mL] false] Water] Insoluble] false Purity: 99.61%
99.61

2-Methoxy-1,4-naphthoquinone Related Products

Signaling Pathway

PKC Signaling Pathways

PKC Signaling Pathway

Choose Selective PKC Inhibitors

Biological Activity

Description 2-Methoxy-1,4-naphthoquinone, isolated from the leaves of Impatiens glandulifera, specifically suppressed the expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells.
Targets
PKCβ1 [1] PKCδ [1] PKCζ [1]
In vitro
In vitro 2-Methoxy-1,4-naphthoquinone (MNQ) exhibits antifungal, antimicrobial activities and showed strong killing effect on K562, HL-60, Raji, MCF-7, H-23, IMR-32, LA-174-T, HSC-2, and SK-MEL-28. MNQ is able to trigger the intrinsic apoptotic pathway in K562 cells, as well as causing p53-dependent cell cycle arrest in the p53-wild type cell line. MNQ inhibits the TCF/β-catenin (TOP) transcriptional activity at IC50 2.9 μM, while it decreases the transcriptional activity of FOP (mutated TCF-binding site)-transfected cell. MNQ exerts an anti-metastatic effect against MDA-MB-231 cells. MNQ has remarkable selectivity towards PKCβI and ζ suppression. MNQ suppresses PKCδ moderately, where MNQ suppresses half of the PKCδ expression at the concentration of 10 μM. MNQ does not suppress PKC βII[1]. MNQ is able to inhibit the invasion and migration characteristics of a highly metastatic MDA-MB-231 cancer cell line[2].
Cell Research Cell lines Raji cells
Concentrations --
Incubation Time 48 h
Method 5×105 Raji cells are incubated in 1 mL of RPMI 1640 medium (supplemented with 10% FBS) containing 0.05 μM PMA, 3 mM SnB and test substance (MNQ or DMSO as the vehicle control) at 37 °C under 5% CO2 for 48 hours. Genistein is used as the standard drug. The cells are then harvested, washed with PBS and fixed onto Teflon-coated slides.

Chemical Information & Solubility

Molecular Weight 188.18 Formula

C11H8O3
CAS No. 2348-82-5 SDF Download 2-Methoxy-1,4-naphthoquinone SDF
Smiles COC1=CC(=O)C2=CC=CC=C2C1=O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 37 mg/mL ( (196.62 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy 2-Methoxy-1,4-naphthoquinone | 2-Methoxy-1,4-naphthoquinone supplier | purchase 2-Methoxy-1,4-naphthoquinone | 2-Methoxy-1,4-naphthoquinone cost | 2-Methoxy-1,4-naphthoquinone manufacturer | order 2-Methoxy-1,4-naphthoquinone | 2-Methoxy-1,4-naphthoquinone distributor