1400W 2HCl

Catalog No.S8337 Synonyms: N-(3-(Aminomethyl)benzyl)acetamidine

1400W 2HCl Chemical Structure

Molecular Weight(MW): 250.17

1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase(iNOS).

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Biological Activity

Description 1400W is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase(iNOS).
Targets
iNOS [1]
(Cell-free assay)
nNOS [1]
(Cell-free assay)
eNOS [1]
(Cell-free assay)
<7 nM(Kd) 2 μM(Ki) 50 μM(Ki)
In vitro

1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS[1].

In vivo 1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%)[1]. 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats[2]. Treatment with 1400W can reduce the rate of growth of solid tumors in mice[4].

Protocol

Cell Research:[3]
+ Expand
  • Cell lines: RAW264.7 cells
  • Concentrations: 100 μM
  • Incubation Time: 16 h
  • Method: RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody.
    (Only for Reference)
Animal Research:[1]
+ Expand
  • Animal Models: Rats
  • Formulation: isotonic saline
  • Dosages: 0.1-10 mg/kg
  • Administration: s.c.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 35 mg/mL (139.9 mM)
Water 35 mg/mL (139.9 mM)
Ethanol 35 mg/mL (139.9 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 250.17
Formula

C10H15N3.2HCl

CAS No. 214358-33-5
Storage powder
Synonyms N-(3-(Aminomethyl)benzyl)acetamidine

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID