1-Deoxynojirimycin

Synonyms: duvoglustat, moranolin

1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.

1-Deoxynojirimycin Chemical Structure

1-Deoxynojirimycin Chemical Structure

CAS: 19130-96-2

Selleck's 1-Deoxynojirimycin has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 98.33%
98.33

1-Deoxynojirimycin Related Products

Choose Selective Carbohydrate Metabolism Inhibitors

Biological Activity

Description 1-Deoxynojirimycin (duvoglustat, moranolin) is a potent α-glucosidase inhibitor and most commonly found in mulberry leaves. It has therapeutic potency against diabetes mellitus.
Targets
α-glucosidase [3]
(Cell-free assay)
In vitro
In vitro 1-Deoxynojirimycin (DNJ) up to 200 μmol/L does not influence survival of HUVECs. DNJ behaves as an antioxidant, decreases ROS production, and delays cellular senescence[2].
Cell Research Cell lines HUVECs
Concentrations 0-200 μmol/L
Incubation Time 24 or 48 h
Method

Cytotoxicity of DNJ is assessed using a WST-8 assay. HUVECs at 80% confluency are trypsinized and transferred to 96-well plates (4000 cells/well). Stock solutions of DNJ are prepared in HuMedia-EG2 medium. DNJ test media are prepared from the stock solutions and diluted to final concentrations of 0-200 μmol/L in HuMedia-EG2 medium. After incubation for 24 h at 37℃, the cells are placed in 200 μL of fresh HuMedia-EG2 medium with various concentrations of DNJ. After 24 or 48 h, 10 μL WST-8 solution is added to each well. After incubation for 3 h at 37℃, cytotoxicity is measured using a microplate reader at a wavelength of 450 nm and a reference wavelength of 655 nm.

In Vivo
In vivo 1-Deoxynojirimycin (DNJ) inhibits intestinal glucose absorption in intestine. 1-Deoxynojirimycin down-regulates intestinal SGLT1, Na+/K+-ATP and GLUT2 mRNA and protein expression. Pretreatment with DNJ (50 mg/kg) increases the activity, mRNA and protein levels of hepatic glycolysis enzymes (GK, PFK, PK, PDE1) and decreases the expression of gluconeogenesis enzymes (PEPCK, G-6-Pase), improves glucose tolerance in both normal and diabetic mice. DNJ inhibits intestinal glucose absorption and accelerates hepatic glucose metabolism by directly regulating the expression of proteins involved in glucose transport systems, glycolysis and gluconeogenesis enzymes[1]. DNJ reduces oxidative stress in the liver and plasma in rodents[2].
Animal Research Animal Models ICR mice
Dosages 50 mg/kg
Administration administered intragastrically
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01380743 Completed
Pompe Disease
Amicus Therapeutics
October 31 2011 Phase 2

Chemical Information & Solubility

Molecular Weight 163.17 Formula

C6H13NO4

CAS No. 19130-96-2 SDF Download 1-Deoxynojirimycin SDF
Smiles C1C(C(C(C(N1)CO)O)O)O
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 33 mg/mL ( (202.24 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 33 mg/mL

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy 1-Deoxynojirimycin | 1-Deoxynojirimycin supplier | purchase 1-Deoxynojirimycin | 1-Deoxynojirimycin cost | 1-Deoxynojirimycin manufacturer | order 1-Deoxynojirimycin | 1-Deoxynojirimycin distributor