Bikinin

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Technical Data

Molecular Weight 273.08 Storage powder
Formula

C9H9BrN2O3

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CAS No. 188011-69-0 Synonyms N/A
Solubility (25°C) * In vitro DMSO 54 mg/mL (197.74 mM)
Water Insoluble
Ethanol Insoluble
In vivo
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Name 4-(5-bromopyridin-2-ylamino)-4-oxobutanoic acid

Biological Activity

Description Bikinin is an ATP-competitive Arabidopsis GSK-3 inhibitor, and acts as a strong activator of brassinosteroid (BR) signaling.
Targets
GSK-3 [1]
In vitro Bikinin directly targets BIN2 of Arabidopsis GSK-3s and activates brassinosteroid-dependent gene expression. [1] Bikinin also inhibits RD26 expression, and inhibits the induction of GUS activity by 150 mM NaCl in the RD26pro:GUS line. [3]
In vivo Bikinin strongly inhibits abscisic acid signaling outputs, and inhibits T180 phosphorylation of SnRK2.3 and its kinase activity in vivo. [2]
Features  

Protocol (Only for Reference)

Kinase Assay:

[1]

Kinase Assays The BIN2 kinase assay with GST-BIN2 and maltose binding protein (MaBP) fused to BES1 (MaBP-BES1) as a substrate is done as reported elsewhere. Kinase assays of the ASKs, AtMPK4 and AtMPK6 with myelin basic protein (MBP), AURORA1 with histone H3, and of the human kinases. Comparable activities of the different ASKs are used. For one particular kinase equal amounts (ranging from 20 to 100 ng depending on the innate kinase activity) are used in the assays with and without bikinin.
 

References

PLEASE KEEP THE PRODUCT UNDER -20°C FOR LONG-TERM STORAGE.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.

Specific storage and handling information for each product is indicated on the product datasheet. Most Selleck products are stable under the recommended conditions. Products are sometimes shipped at a temperature that differs from the recommended storage temperature. Short-term storage of many products are stable in the short-term at temperatures that differ from that required for long-term storage.
We ensure that the product is shipped under conditions that will maintain the quality of the reagents. Upon receipt of the product, follow the storage recommendations on the product data sheet.

Chemical Structure

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