The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL
The antiserum was produced against synthesized phosphopeptide derived from human STAT5A around the phosphorylation site of tyrosine 694 (D-G-Yp-V-K).
STAT5A (phospho-Tyr694) antibody detects endogenous levels of STAT5A only when phosphorylated at tyrosine 694.
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, Store at -20°C. Stable for one year from the date of shipment.
Human, Mouse, Rat
Use an anti-rabbit secondary antibody to detect the antibody.
|Western blot||1/500 - 1/1000|
|Immunohistochemistry||1/50 - 1/100|
|Not yet tested in other applications|
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT5A (phospho-Tyr694) antibody
Western blot analysis of extracts from HeLa cells using STAT5A (phospho-Tyr694) antibody