Inhibitor Expert (Inhibitors, Modulators, Libraries)
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This gene encodes an intracellular tyrosine kinase expressed in T-cells. The protein contains both SH2 and SH3 domains which are often found in intracellular kinases. It is thought to play a role in T-cell proliferation and differentiation.
Purified recombinant fragment of human ITK expressed in E. Coli.
ITK mouse mAb detects endogenous levels of ITK protein
Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment.
Human
Mouse IgG1
Use an anti-mouse secondary antibody to detect the antibody.
72 kDa
| Western blot | 1:500 - 1:1000 |
| Immunohistochemistry | 1:200 - 1:1000 |
| Flow cytometry | 1:200 - 1:400 |
| Enzyme-linked Immunosorbent assay | 1:10000 |
| Not yet tested in other applications | |
Western blot analysis using ITK mAb against recombinant protein. |
Western blot analysis using ITK mouse mAb against Jurkat cell lysate. |
Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. |
Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red). |
ELISA analysis using ITK mAb against recombinant protein. |