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WIF1 Antibody (1G5)

Mouse Monoclonal Antibody
Size Price Quantity
100μl USD 250
Clonality: Mouse Monoclonal Antibody
Isotype: Mouse IgG1
Clone Number: 1G5
Reactivity: Human
Molecular Weight: 42 kDa
Swiss-Prot:

Q9Y5W5

Sensitivity: WIF1 Mouse mAb detects endogenous levels of WIF1 protein.
Immunogen:

Purified recombinant fragment of human WIF1 expressed in E. coli.

Storage: Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment.
Synonyms: WIF antibody; WIF-1wnt inhibitory factor 1 antibody

Tested Applications

Applications Recommended Dilutions Protocols
Western blot 1:500 - 1:2000
Immunohistochemistry 1:200 - 1:1000
Immunofluorescence 1:200 - 1:1000
Enzyme-linked Immunosorbent assay 1:10000

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Application Data

  • Western blot analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
  • Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.
  • Immunofluorescence analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Background

WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity [1]. It contains an N-terminal WIF domain and five EGF-like repeats [2]. The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion [3,4]. It has been reported that WIF1 expression is downregulated in many types of cancers [5-8].

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