Western blot analysis using WIF1 mouse mAb against Hela (1), NIH/3T3 (2) and NTERA-2 (3) cell lysate.
Immunohistochemical analysis of paraffin-embedded ovary tumour tissues (left) and lung cancer (right) using WIF1 mouse mAb with DAB staining.
Immunofluorescence analysis of Hela cells using WIF1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
WIF1 (Wnt inhibitory factor 1) is a secreted protein that binds to Wnt proteins and inhibits their activity . It contains an N-terminal WIF domain and five EGF-like repeats . The WIF1 ortholog in Drosophila, Shifted, is required for Hedgehog stability and diffusion [3,4]. It has been reported that WIF1 expression is downregulated in many types of cancers [5-8].