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PTH1R Antibody (4D2)

Mouse Monoclonal Antibody
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100μl USD 250
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Product Information
PTH1R Antibody (4D2) Antibody Datasheet
Clonality: Mouse Monoclonal Antibody
Isotype: Mouse IgG1
Clone Number: 4D2
Reactivity: Human
Molecular Weight: 66 kDa
Swiss-Prot:

Q03431
Sensitivity: PTH1R Mouse mAb detects endogenous levels of PTH1R protein.
Immunogen:

Purified recombinant fragment of human PTH1R expressed in E. coli.
Storage: Supplied in PBS, 0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment.
Synonyms: MGC138426 antibody; MGC138452 antibody; PFE antibody; PTHR antibody; PTHR1PTH/PTHr receptor antibody; PTH/PTHrP type I receptor antibody; PTH1 receptor antibody; parathyroid hormone receptor 1 antibody; parathyroid hormone/parathyroid hormo

Tested Applications

Applications Recommended Dilutions Protocols
Western blot 1:500 - 1:2000
Immunohistochemistry 1:200 - 1:1000
Immunofluorescence 1:200 - 1:1000
Enzyme-linked Immunosorbent assay 1:10000

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Application Data

  • Western blot analysis using PTH1R mAb against PTH1R-hIgGFc transfected HEK293 cell lysate.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissues using PTH1R mouse mAb with DAB staining.
  • Immunofluorescence analysis of SK-BR-3 cells using PTH1R mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Background

PTH1R (parathyroid receptor­1, also called PTHR1) is an 85 ­ 95 kDa member of the G­protein coupled receptor family 2 that is a critical receptor regulating Ca++ homeostasis [1 - 5]. The human PTH1R cDNA encodes 593 amino acids (aa) including a 26 aa signal sequence and a long extracellular domain (aa 27 - 189) that contains the PTH (parathyroid hormone) and PTHrP (PTH-related protein) docking site (aa 173 - 189), followed by seven transmembrane domains within the C­ terminal 404 aa [2 - 4]. Within the N­ terminal ECD, human PTH1R shares 87%, 88%, 96%, 94% and 93% aa identity with mouse, rat, canine, bovine and porcine PTH1R, respectively. PTH of other mammals will bind and stimulate human PTH1R, although with differing affinities [2]. PTH1R is mainly expressed in bone and kidney, but is also present on hepatocytes, smooth muscle cells, and in other tissues where PTH and PTHrP are found [1 ­ 3, 5, 6]. Through PTH1R on osteoblasts, PTH promotes differentiation of osteoclasts, which in turn promote release of Ca++ from bone [1, 5]. PTH1R on osteocytes, however, allows PTH to promote bone formation [7]. In renal epithelium, it promotes conversion of Vitamin D to its active form, lowers Ca++ excretion and increases phosphate excretion [1, 5]. Following PTH binding, PTH1R undergoes a conformation change which activates cAMP, IP3, PKC and Ca++ signaling pathways [1, 8]. PTH1R is then phosphorylated and down-regulated by internalization [9]. Dwarfism in Jansen­type metaphyseal chondrodysplasia is associated with PTH1R gain of function, while in Blomstrand chondrodysplasia, PTH1R function is lost [10, 11]. Impaired PTH1R function may be a factor in developmental endochondromas in Ollier disease [12]. Polymorphisms may also be associated with variation in bone mineral density [13].

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