Myc (phospho-Thr358) antibody detects endogenous levels of Myc only when phosphorylated at threonine 358.
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of threonine 358 (R-R-Tp-H-N).
Storage:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, Store at -20°C. Stable for one year from the date of shipment.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Myc (phospho- Thr358) antibody
Western blot analysis of extracts from HT-29 cells treated with UV (20min), using Myc (Ab-358) antibody (Lane 1 and 2) and Myc (phospho-Thr358) antibody (Lane 3 and 4)
Background
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
