LIMK2 (phospho-Thr505) antibody detects endogenous levels of LIMK2 only when phosphorylated at threonine 505.
Immunogen:
The antiserum was produced against synthesized phosphopeptide derived from human LIMK2 around the phosphorylation site of threonine 505 (R-Y-Tp-V-V).
Storage:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, Store at -20°C. Stable for one year from the date of shipment.
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using LIMK2 (phospho-Thr505) antibody
Western blot analysis of extract from NIH/3T3 cells treated or untreated with UV (60min), using LIMK2 (phospho- Thr505) antibody
Background
LIM-kinase 2 (LIMK2), a 75-kDa member of the LIM kinase family, is responsible for regulating actin polymerization via phosphorylation and inactivation of the actin depolymerizing factors ADF/cofilin . LIMK2 protein is found in the cytoplasm during interphase, redistributed to the mitotic spindle and then to the spindle midzone during anaphase to telophase (2). It has been shown that ROCK, a downstream protein kinase of Rho, activates LIMK2 by directly phosphorylates threonine 505 residue. Since activity of LIMK2 is cell cycle dependant, LIMK2 activity is markedly increased when HeLa cells are synchronized at mitosis with nocodazole treatment.
