Chk1 antibody detects endogenous levels of total Chk1 protein.
Immunogen:
The antiserum was produced against synthesized non-phosphopeptide derived from human Chk1 around the phosphorylation site of serine 317 (S-S-Sp-Q-P)
Storage:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, Store at -20°C. Stable for one year from the date of shipment.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Chk1 antibody .
Western blot analysis of the extracts using Chk1 antibody
Background
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.