Western blot analysis of HeLa cell lysate, using beta-Tubulin (10B1) Mouse mAb. The antibody dilutions are 1:2000 (lane 1), 1:5000(lane 2) and 1:10000 (lane 3). Each lane was loaded with 10 μg of cell lysate.
Immunofluorescence analysis of HeLa cells, NIH/3T3 cells, COS-7 cells and 293T cells, using beta-Tubulin (10B1) Mouse mAb at a 1:1000 dilution.
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Tubulin is a major cytoskeleton component that has five distinct forms, designated α-, β-, γ-, δ-, and ε-Tubulin. α- and β-Tubulins form heterodimers which multimerize to form a microtubule filament. There are five β-Tubulin isoforms (β1, β2, β3, β4A and β4B) that are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ-Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ-Tubulin and ε-Tubulin are associated with the centrosome. δ-Tubulin is a homolog of the Chlamydomonas δ-Tubulin Uni3 and is found in association with the centrioles, whereas ε-Tubulin localizes to the pericentriolar material. ε-Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.
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