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beta-Actin Antibody (8H10D10)

Mouse Monoclonal Antibody
Size Price Quantity
100μl USD 250
1ml USD 800
Clonality: Mouse Monoclonal Antibody
Isotype: Mouse IgG2b
Clone Number: 8H10D10
Reactivity: Human, Mouse, Hamster, Rat, Monkey
Molecular Weight: 42 kDa
Swiss-Prot:

P60709

Sensitivity: β-Actin Mouse mAb detects endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.
Immunogen:

Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.

Storage: Supplied in PBS,0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment.
Synonyms: ACTB antibody; PS1TP5BP1PS1TP5-binding protein 1 antibody; actin, cytoplasmic 1 antibody; beta cytoskeletal actin antibody; actin, beta antibody

Tested Applications

Applications Recommended Dilutions Protocols
Western blot 1:500 - 1:2000
Immunofluorescence 1:200 - 1:1000
Flow cytometry 1:200 - 1:400
Enzyme-linked Immunosorbent assay 1:10000

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Application Data

  • Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
  • Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta-Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
  • Flow cytometric analysis of MCF-7 cells using beta-Actin mouse mAb (right) and negative control (left).

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility [1]. α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells [1]. Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin [2]. The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments [2]. It has been reported that actin is hyperphosphorylated in primary breast tumors [3]. Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle [4-6]. Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis [6].

References

  • 1. Herman, I.M. Curr. Opin. Cell Biol. 1993; 5: 48-55.<
    2. Condeelis, J. Trends Cell Biol. 2001; 11: 288-293.
    3. Lim, Y.P. et al. Clin. Cancer Res. 2004; 10: 3980-3987.
    4. Kayalar, C. et al. Proc. Natl. Acad. Sci. USA. 1996; 93: 2234-2238.
    5. Communal, C. et al. Proc. Natl. Acad. Sci. USA. 2002; 99: 6252-6256.
    6. Du, J. et al. J. Clin. Invest. 2004; 113: 115-123.

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