β-Actin Mouse mAb detects endogenous levels of total β-actin protein. This antibody may cross-react with the γ-actin (cytoplasmic isoform). It does not cross-react with α-skeletal, α-cardiac, α-vascular smooth, or γ-enteric smooth muscle isoforms.
Synthetic peptide corresponding to amino-terminal residues of human beta-Actin, conjugated to KLH.
Supplied in PBS,0.005% sodium azide, PH 7.4. Store at -20°C. Stable for one year from the date of shipment.
Western blot analysis using beta-Actin mouse mAb against NIH/3T3 (1), Jurkat (2), HeLa (3), CHO (4), PC12 (5), HEK293 (6), COS (7), A549 (8) and MCF-7 (9) cell lysate.
Confocal immunofluorescence analysis of SKBR-3 (left) and A549 (right) cells using beta-Actin mouse mAb (red, the secondary Ab is Cy3-Goat anti mouse IgG). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric analysis of MCF-7 cells using beta-Actin mouse mAb (right) and negative control (left).
Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility . α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells . Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin . The Arp2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments . It has been reported that actin is hyperphosphorylated in primary breast tumors . Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle [4-6]. Actin cleavage by caspase-3 may accelerate ubiquitin/proteosome dependent muscle proteolysis .
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