Western blot analysis using ATF2 mouse mAb against NIH/3T3 cell lysate.
Immunohistochemical analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using ATF2 mouse mAb with DAB staining.
ELISA analysis using ATF2 mouse mAb.
Background
The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins [1]. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways [2-4]. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71 [2-4]. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2 [2-4]. In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2 [2].
