Cat.NO: A1080
PARP Antibody
Mouse mAb
Synonyms: PARP; PPOL; ADPRT; ADPRT1; PARP-1; pADPRT-1; PARP1
Swiss Prot: P09874
Product Information
Reactivity: Human
MW: 117 kDa
Sensitivity: PARP Mouse mAb detects endogenous levels of PARP protein.
Concentration: 1 mg/ml
Applications: Recommended dilutions: Western Blotting 1:500 - 1:2000 FlowCyt 1:200 - 1:400 |
Product Data
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Western blot
Western blot analysis using PARP mouse mAb against Jurkat (1), K562 (2), HeLa (3), Raji (4),THP-1 (5) and SW620 (6) cell lysate.
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Flow cytometry
Flow cytometric analysis of Jurkat cells using anti-PARP mAb (green) and negative control (purple).
Background
PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress [1]. This protein can be cleaved by many ICE-like caspases in vitro [2,3] and is one of the main cleavage targets of caspase-3 in vivo [4,5]. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) [2,4]. PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis [6].
References
1. Satoh, M.S. and Lindahl, T. Nature 1992; 356: 356-358.
2. Lazebnik, Y. A. et al. Nature 1994; 371: 346-347.
3. Cohen, G.M. Biochem. J. 1997; 326: 1-16.
4. Nicholson, D. W. et al. Nature 1995; 376: 37-43.
5. Tewari, M. et al. Cell 1995; 81: 801-809.
6. Oliver, F.J. et al. J. Biol. Chem. 1998; 273: 33533-33539.
Protocol
Western Blot
Flow Cytometry
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