Cat.NO: A1003
Myc-Tag Antibody (19C2)
Mouse mAb
Data sheet: 
PDF
Synonyms: MYC; MRTL; bHLHe39; c-Myc; Myc proto-oncogene protein; Proto-oncogene c-Myc; Transcription factor p64
Swiss Prot:
Product Information
Reactivity: Human,Mouse,Rat
MW:
Sensitivity: Myc-tag (19C2) mouse mAb detects over-expressed or recombinant proteins containing the Myc epitope tag.
Concentration: 1 mg/ml
Applications: Recommended dilutions: Western Blotting 1:5000 IP 1:200 IF 1:2000 |
Product Data
Western blot analysis of over-expressed Myc-tagged proterin in 293T cell lysate, using Myc-tag (19C2) Mouse mAb. The antibody dilutions are 1:5000 (lane 1) and 1:10000(lane 2). Each lane was loaded with 10 μg of cell lysate.
.gif "IF analysis of 293T cells transfected (left) with a Myc-tagged protein and a mock transfection (ringht, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb at a 1:2000 dilution")
IF analysis of 293T cells transfected (left) with a Myc-tagged protein and a mock transfection (ringht, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb at a 1:2000 dilution
IP of extracts from 293T cells transfected (lane 1) with a Myc-tagged protein and a mock transfection (lane 2, using the same protein without the Myc tag), using Myc-Tag (19C2) Mouse mAb and probed on Western blot using the same antibody. Dulition:1:200(1), 1:500(2), 1:200(3)."
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein’s biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian cell systems.
References
1. Munro, S. and Pelham. EMBO J. 1984 Dec 20; 3(13): 3087-3093.
Protocol
Western Blot
Immunoprecipitation
Immunofluorescence
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