Western blot analysis of Hela whole cell lysate using GAPDH Antibody, The dilutions are 1:1000 (lane 1), 1:2000 (lane 2), 1:5000 (lane 3). Each lane was loadd with 20 μg of Hela cell lysate.
Western blot analysis of lysates from various cell lines using GAPDH Antibody (1:1000). Each lane was loaded with 20 μg whole cell lysate. Lane 1:Hela; Lane 2: 293T; Lane 3:NIH/3T3; Lane 4: COS7.
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Western blot analysis of lysate from cell line using GAPDH mAb(1:1000). Lane1:NIH cell.GAPDH antibody was supplied by Selleck. Data were provided by Dr Zhang of Tianjin Medical University.
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Background
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. Though differentially expressed from tissue to tissue, GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets. Recent work has elucidated roles for GAPDH in apoptosis, gene expression and nuclear transport. GAPDH may also play a role in neurodegenerative pathologies such as Huntington and Alzheimer’s diseases.
References
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3. Zheng, L. et al. Cell. 2003 7 25; 114(2): 255-66.
4. Bae, B.I. et al. Proc. Natl. Acad. Sci. 2006 2 28; 103(9): 3405-9
5. Wang, Q. et al. FASEB J. 2005 3; 19: 869-71.
