PARP Rabbit Recombinant mAb

Catalog No.A5037

Size Price Stock Quantity  
USD 97 In stock
USD 157 In stock
USD 547 In stock

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

  1. 1. Why choose rabbit monoclonal antibody?
  2. Rabbit monoclonal antibody has over 100 times higher affinity than that of mouse monoclonal.
  3. 2. Why choose recombinant antibodies?
  4. Recombinant antibodies are known for higher purity and minimal deviation between batches, versus regular antibodies.
  • WB

Usage Information

Application WB
Dilution
WB
1:1000
Reactivity Human Mouse Rat
MW (kDa) 113 kDa
Source Rabbit
Concentration 1mg/ml
Storage buffer 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide.
Storage Store at –20°C.

Protocol

WB
+ Expand

Western Blotting

Sample preparation

1. Aspirate media from cultures and Wash the cells with 1X PBS.
2. Lyse cells by adding 1X SDS sample buffer and transfer the extract to a microcentrifuge tube. Keep onice.
3. Sonicate for 10–15 sec to complete cell lysis and shear DNA.
4. Heat a 20 µl sample to 95–100°C for 5 min, then cool on ice.
5. Centrifuge for 5 min (with Microcentrifuge).
6. Load appropriate volumes of samples onto SDS-PAGE gel (loading quantity of protein sample depends on the concentration of extracted proteins).
NOTE: At the same time, please load the pre-stained molecular weight markers to determine molecular weights and verify electrotransfer.
7. Electrotransfer to nitrocellulose/PVDF membrane.

Membrane Blocking and Antibody Incubations

a. Blocking

1. (Optional) After transfer, wash the transferred membrane with TBS for 5 min at room temperature.
2. Incubate the membrane in the blocking buffer for 1 hr at room temperature.
3. Wash three times for 5 min each with TBST.

b. Antibodies Incubation

1. Incubate membrane and primary antibody (at the appropriate dilution and diluent recommended) in a primary antibody dilution buffer with gentle agitation overnight at 4°C.
2. Wash three times for 5 min each with TBST.
3. Incubate membrane with an appropriate second antibodydissolved in the blocking buffer with gentle agitation for 1 hr at room temperature.
4. Wash three times for 5 min each with TBST.
5. Proceed with detection.

Detection of Proteins

1. After antibodies incubation, Wash membrane three times for 5 minutes in TBST.
2. PrepareECL Reagent (or other chromogenic agents/substrate according to your second antibody). Mix well.
3. Incubate substrate with membrane for 1 minute, remove excess solution (membrane remains wet), wrap in plastic and expose to X-ray film.

Description

Specificity

PARP Rabbit Recombinant mAb detects endogenous levels of PARP.

Background

Poly (ADP-ribose) polymerase (PARP) is a family of proteins involved in a number of cellular processes such as DNA repair, genomic stability, and programmed cell death. PARPs have the ability to catalyze the transfer of ADP-ribose to target proteins. There are at least 18 members of the PARP family that are encoded by different genes, and share homology in a conserved catalytic domain. A number of cellular substrates for PARP have been defined, and a majority of these proteins are nuclear proteins that are involved in nucleic acid metabolism, modulation of chromatin structure, DNA synthesis, and DNA repair. It plays a central role in NER and BER, and enables repair of DNA damage caused by alkylating agents and chemotherapeutic drugs.

Datasheet & MSDS

Related Antibodies

PARP Signaling Pathway Map

Related PARP Products

Tags: buy PARP Rabbit Recombinant mAb | PARP Rabbit Recombinant mAb supplier | purchase PARP Rabbit Recombinant mAb | PARP Rabbit Recombinant mAb cost | PARP Rabbit Recombinant mAb manufacturer | order PARP Rabbit Recombinant mAb | PARP Rabbit Recombinant mAb distributor